Application
Western blot analysis of CYP7A1 Antibody (C-term) in K562(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). CYP7A1 (arrow) was detected using the purified Pab.| Product Name | CYP7A1 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CYP7A1 (C-term)This gene encodes a member of the cytochrome P45 superfamily of enzymes. The cytochrome P45 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 475~504 amino acids from the C-terminal region of human CYP7A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Cholesterol 7-alpha-hydroxylase CYP7A1 Cholesterol7alphamonooxygenase Cholesterol7alphahydroxylase Cytochrome P450 7A1 CYPVII CYP7 Cholesterol 7-alpha-monooxygenase Cytochrome P4507A1 Cholesterol 7alphamonooxygenase Cholesterol 7alphahydroxylase |
|---|---|
| Target | CYP7A1 |
| Molecular Weight(MW) | 57.661 kDa (note) |
Application
Western blot analysis of CYP7A1 Antibody (C-term) in K562(lane 1), HepG2(lane 2) cell line lysates (35ug/lane). CYP7A1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
