Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1500Application
CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human liver carcinoma. CYP7B1 antibody [C3], C-term diluted at 1:500.Application
CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP7B1 protein stained by CYP7B1 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | CYP7B1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CYP7B1 (cytochrome P450, family 7, subfamily B, polypeptide 1)This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CYP7B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CBAS3 antibody CP7B antibody SPG5A antibody CYP7B1 antibody cytochrome P450 7B1 antibody oxysterol 7-alpha-hydroxylase antibody 25-hydroxycholesterol 7-alpha-hydroxylase antibody "cytochrome P450 subfamily VIIB (oxysterol 7 alpha-hydroxylase) poly |
|---|---|
| Target | CYP7B1 |
| Molecular Weight(MW) | 58 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane , Microsome membrane |
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 10% SDS PAGE diluted at 1:1500
Application
CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human liver carcinoma. CYP7B1 antibody [C3], C-term diluted at 1:500.
Application
CYP7B1 antibody [C3], C-term detects CYP7B1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP7B1 protein stained by CYP7B1 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
