Application
CYR61 antibody [N1C3] detects CYR61 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. CYR61 antibody [N1C3] diluted at 1:500.Application
CYR61 antibody [N1C3] detects CYR61 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYR61 protein stained by CYR61 antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining.Application
CYR61 antibody detects CYR61 protein by western blot analysis. Un-treated (-) and treated (+, 1x Brefeldin A treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody diluted by 1:1000.| Product Name | CYR61 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CYR61 (cysteine-rich, angiogenic inducer, 61)CYR61 is a secreted, cysteine-rich, heparin-binding protein encoded by a growth factor-inducible immediate-early gene. Acting as an extracellular, matrix-associated signaling molecule, CYR61 promotes the adhesion of endothelial cells through interaction with integrin and augments growth factor-induced DNA synthesis in the same cell type.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYR61. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Chicken, Chimpanzee, BovineMouse (91%), Chicken (82%), Chimpanzee (99%), Bovine (92%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCN1 antibody GIG1 antibody IGFBP10 antibody CYR61 antibody CCN family member 1 antibody insulin-like growth factor-binding protein 10 antibody "cysteine-rich anigogenic inducer 61 antibody" IGFBP-10 antibody cysteine-rich heparin-binding protein |
|---|---|
| Target | CYR61 |
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Secreted |
Application
CYR61 antibody [N1C3] detects CYR61 protein at cytoplasm in rat intestine by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. CYR61 antibody [N1C3] diluted at 1:500.
Application
CYR61 antibody [N1C3] detects CYR61 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYR61 protein stained by CYR61 antibody [N1C3] diluted at 1:200. Blue: Hoechst 33342 staining.
Application
CYR61 antibody detects CYR61 protein by western blot analysis. Un-treated (-) and treated (+, 1x Brefeldin A treatment for 24 hrs) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody diluted by 1:1000.| Positive Control | HeLa (1X Brefeldin A for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
