Rabbit polyclonal antibody to Cytochrome b5The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Immunogen
Recombinant protein of human CYB5A
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
WB: 1:500 - 1:2000. IHC: 1:50 - 1:200:
Species Reactivity
HumanMouseRabbit
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CYB5 cytochrome b5 type A (microsomal) CYB5A MCB5
Target
Cytochrome b5
Molecular Weight(MW)
15 kDa (note)
Application
Application
WB analysis of extracts from various cell lines using Cytochrome b5 antibody.
Application
IHC-P analysis of human colon tissue using Cytochrome b5 antibody at a dilution of 1:200.
Application Notes
WB: 1:500 - 1:2000. IHC: 1:50 - 1:200:
Additional Information
Form
Liquid
Storage Instructions
Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.