Application
Western blot analysis of cytochrome c in Jurkat (left lane) and 3T3 (right lane) cell lysates.| Product Name | Cytochrome C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cytochrome cCytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.: |
|
| Species Reactivity | HumanMouseChickenDogRatBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYC CYCS |
|---|---|
| Target | Cytochrome c |
| Tissue Specificity | This antibody is specific for Cytochrome c. |
Application
Western blot analysis of cytochrome c in Jurkat (left lane) and 3T3 (right lane) cell lysates.| Application Notes | Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
