Application
Western Blot analysis shows that rabbit anti-cytochrome c binds to a band at MW about 12KDa and a band at about 24KDa (doublet cytochrome c) on rat PC12 cell lysate (30 ug).| Product Name | Cytochrome C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Anti-Cytochrome C polyclonal antibodyThis gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome. |
| Immunogen | Bovine Cytochrome C |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| The product can be used at 1:2,000 to 1:10,000 for both ELISA and immunoblot testing. Do not use dry milk in blocking or dilution beffer in your assay. Please use BSA (3-5% in blocking and 1-3% in dilution buffers) in your assay. User needs to try out the tiration for immunohistochemical testing.: |
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| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYC 123970 54205 Cytochrome C P99999 HCS CYCS MGC128356 |
|---|---|
| Target | Cytochrome C |
Application
Western Blot analysis shows that rabbit anti-cytochrome c binds to a band at MW about 12KDa and a band at about 24KDa (doublet cytochrome c) on rat PC12 cell lysate (30 ug).| Application Notes | The product can be used at 1:2,000 to 1:10,000 for both ELISA and immunoblot testing. Do not use dry milk in blocking or dilution beffer in your assay. Please use BSA (3-5% in blocking and 1-3% in dilution buffers) in your assay. User needs to try out the tiration for immunohistochemical testing.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 0.02% Na Azide |
