Application
CYP4F11 antibody [N1N3] detects CYP4F11 protein at cytoplasm on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung adenocarcinoma. CYP4F11 antibody [N1N3] dilution: 1:500.Application
CYP4F11 antibody [N1N3] detects CYP4F11 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP4F11 protein stained by CYP4F11 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Product Name | Cytochrome P450 4F11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytochrome P450 4F11This gene, CYP4F11, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F2, is approximately 16 kb away. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CYP4F11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYPIVF11 antibody CYP4F11 antibody cytochrome P450 4F11 antibody "cytochrome P450 subfamily IVF polypeptide 11 antibody" "cytochrome P450 family 4 subfamily F polypeptide 11 antibody" |
|---|---|
| Target | Cytochrome P450 4F11 |
| Molecular Weight(MW) | 60 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein , Microsome membrane; Single-pass membrane protein , Endoplasmic reticulum membrane , Microsome membrane |
Application
CYP4F11 antibody [N1N3] detects CYP4F11 protein at cytoplasm on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung adenocarcinoma. CYP4F11 antibody [N1N3] dilution: 1:500.
Application
CYP4F11 antibody [N1N3] detects CYP4F11 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CYP4F11 protein stained by CYP4F11 antibody [N1N3] diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
