Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Cytokeratin 13 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using Cytokeratin 13 antibody at 1:500 dilution.| Product Name | Cytokeratin 13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 13 (keratin 13)The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Cytokeratin 13. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK13 antibody K13 antibody MGC161462 antibody MGC3781 antibody KRT13 antibody cytokeratin 13 antibody keratin-13 antibody "keratin type I cytoskeletal 13 antibody" CK-13 antibody cytokeratin-13 antibody keratin 13 antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using Cytokeratin 13 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using Cytokeratin 13 antibody at 1:500 dilution.| Positive Control | MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
