Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Cytokeratin 17 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 17 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE Cytokeratin 17 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cytokeratin 17 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 17 (keratin 17)This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 17. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Sheep, Chimpanzee, BovineMouse (94%), Rat (94%), Sheep (92%), Chimpanzee (100%), Bovine (91%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | K17 antibody PC antibody PC2 antibody PCHC1 antibody KRT17 antibody CK-17 antibody keratin 17 epitope S1 antibody keratin 17 epitope S4 antibody 39.1 antibody keratin 17 epitope S2 antibody "keratin type I cytoskeletal 17 antibody" cytokeratin- |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Cytokeratin 17 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 17 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE Cytokeratin 17 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
