Application
Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18 antibody at 1:100 dilution.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal diluted at 1:2000.Application
Sample(30 μg of whole cell lysate) A:H1299 B:Hep G2 7.5% SDS PAGE diluted at 1:3000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 antibody at 1:200 dilution.Application
Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Cytokeratin 18 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Cytokeratin 18 (keratin 18)KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 18. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, BovineMouse (93%), Rat (92%), Dog (92%), Bovine (92%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYK18 antibody K18 antibody KRT18 antibody cytokeratin-18 antibody cell proliferation-inducing gene 46 protein antibody cytokeratin 18 antibody keratin-18 antibody cell proliferation-inducing protein 46 antibody "keratin type I cytoskeletal 18 ant |
|---|---|
| Target | Cytokeratin 18 |
| Molecular Weight(MW) | 48 kDa (note) (2) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
Immunohistochemical analysis of paraffin-embedded human endo mitral ovarian cancer, using KRT18 antibody at 1:100 dilution.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 18 antibody [N2C2], Internal diluted at 1:2000.
Application
Sample(30 μg of whole cell lysate) A:H1299 B:Hep G2 7.5% SDS PAGE diluted at 1:3000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 18 antibody at 1:200 dilution.
Application
Cytokeratin 18 antibody [N2C2], Internal detects Cytokeratin 18 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytokeratin 18 protein stained by Cytokeratin 18 antibody [N2C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , H1299 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
