Rabbit Polyclonal antibody to Cytokeratin 18 (phospho Ser52)KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cytokeratin 18. The exact sequence is proprietary.
Modification
p-Ser52
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
keratin 18 type I K18 KRT18 CYK18
Target
Cytokeratin 18 (phospho Ser52)
Tissue Specificity
Recognizes endogenous levels of Cytokeratin 18 (pS52) protein.
Application
Application
Western blot analysis of Cytokeratin 18 (pS52) expression in HeLa (A); MCF7 (B); NIH3T3 (C); PC12 (D) whole cell lysates.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.