Application
Western blot analysis of extracts from 293 cells using Keratin 18(Ab-33) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Ab-33) Antibody (left) or the same antibody preincubated with blocking peptide (right)| Product Name | Cytokeratin 18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 18KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. |
| Immunogen | Peptide sequence around aa.31~35 (A-A-S-V-Y) derived from Human Keratin 18 (CK18). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CYK18 K18 KRT18 cytokeratin-18 cell proliferation-inducing gene 46 protein cytokeratin 18 keratin-18 cell proliferation-inducing protein 46 keratin type I cytoskeletal 18 CK-18 keratin 18 |
|---|---|
| Target | Cytokeratin 18 |
| Molecular Weight(MW) | 46 (note) |
Application
Western blot analysis of extracts from 293 cells using Keratin 18(Ab-33) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 18(Ab-33) Antibody (left) or the same antibody preincubated with blocking peptide (right)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
