Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 19 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded OVCA, using Cytokeratin 19 antibody at 1:500 dilution.| Product Name | Cytokeratin 19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 19 (keratin 19)The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Chicken, BovineMouse (83%), Rat (81%), Dog (93%), Chicken (87%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK19 antibody K19 antibody K1CS antibody MGC15366 antibody KRT19 antibody cytokeratin-19 antibody cytokeratin 19 antibody "keratin type I 40-kd antibody" keratin-19 antibody "keratin type I cytoskeletal 19 antibody" 40-kDa keratin intermediate |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 19 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded OVCA, using Cytokeratin 19 antibody at 1:500 dilution.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
