Application
Immunohistochemical analysis of paraffin-embedded Gastric ca, using Cytokeratin 31 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:5000Application
Cytokeratin 31 antibody [N3C3] detects KRT31 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT31 protein stained by Cytokeratin 31 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Cytokeratin 31 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 31 (keratin 31)The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 31. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (88%), Rat (85%), Pig (86%), Chimpanzee (100%), Bovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HA1 antibody Ha-1 antibody KRTHA1 antibody MGC138630 antibody hHa1 antibody KRT31 antibody keratin-31 antibody "hair keratin type I Ha1 antibody" "hard keratin type I 1 antibody" "keratin type I cuticular Ha1 antibody" K31 antibody "keratin |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded Gastric ca, using Cytokeratin 31 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:5000
Application
Cytokeratin 31 antibody [N3C3] detects KRT31 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KRT31 protein stained by Cytokeratin 31 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
