Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 33B antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 33B antibody at 1:500 dilution.| Product Name | Cytokeratin 33B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 33B (keratin 33B)The protein encoded by this gene is a member of the keratin gene family. It is one of the type I hair keratin genes which are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 33B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, DogMouse (89%), Rat (90%), Dog (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HA3II antibody Ha-3II antibody KRTHA3A antibody KRTHA3B antibody hHa3-II antibody KRT33B antibody keratin-33B antibody "hard keratin type I 3II antibody" "keratin type I cuticular Ha3-II antibody" "hair keratin type I Ha3-II antibody" type I |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 33B antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cytokeratin 33B antibody at 1:500 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
