Application
Sample(30 µg of whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using KRT6A antibody at 1:100 dilution.Application
Cytokeratin 6 antibody detects Cytokeratin 6 protein at membrane on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Cytokeratin 6 antibody diluted at 1:500.| Product Name | Cytokeratin 6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to keratin 6A (keratin 6A)The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK6A antibody CK6C antibody CK6D antibody K6A antibody K6C antibody K6D antibody KRT6C antibody KRT6D antibody KRT6A antibody cytokeratin 6D antibody cytokeratin 6C antibody "keratin epidermal type II K6A antibody" type-II keratin Kb6 antibod |
|---|---|
| Target | keratin 6A |
| Molecular Weight(MW) | 60 kDa (note) |
Application
Sample(30 µg of whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded D54MG xenograft, using KRT6A antibody at 1:100 dilution.
Application
Cytokeratin 6 antibody detects Cytokeratin 6 protein at membrane on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Cytokeratin 6 antibody diluted at 1:500.| Positive Control | A431 , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
