Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 7 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7 antibody at 1:500 dilution.| Product Name | Cytokeratin 7 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 7 (keratin, type II cytoskeletal 7)The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (88%), Rat (88%), Chicken (80%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK7 antibody K2C7 antibody K7 antibody MGC129731 antibody MGC3625 antibody SCL antibody KRT7 antibody "keratin 55K type II cytoskeletal antibody" cytokeratin 7 antibody cytokeratin-7 antibody CK-7 antibody "keratin type II cytoskeletal 7 antib |
|---|---|
| Molecular Weight(MW) | 51 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Cytokeratin 7 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Cytokeratin 7 antibody at 1:500 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
