Application
Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8 (Phospho-Ser74) AntibodyApplication
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8 (Phospho-Ser74) Antibody| Product Name | Cytokeratin 8 (phospho Ser74) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 8 (phospho Ser74)Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. |
| Immunogen | Peptide sequence around phosphorylation site of serine 74 (L-L-S(p)-P-L) derived from Human Keratin 8 (CK8). |
| Modification | p-Ser74 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CARD2 CK8 CYK8 K2C8 K8 KO KRT8 cytokeratin-8 type-II keratin Kb8 cytokeratin 8 keratin type II cytoskeletal 8 keratin 8 |
|---|---|
| Target | Cytokeratin 8 (phospho Ser74) |
| Molecular Weight(MW) | 55 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using Keratin 8 (Phospho-Ser74) Antibody
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using Keratin 8 (Phospho-Ser74) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
