Application
Western blot analysis of extracts of various cell lines, using KRT8 antibody.Application
Immunofluorescence analysis of A549 cell using KRT8 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of HeLa cell using KRT8 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human esophageal using KRT8 antibody at dilution of 1:100 (x400 lens).| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 8This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] |
| Immunogen | Recombinant protein of human KRT8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:100, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYK8 CK8 CARD2 CK-8 K8 KRT8 keratin 8 type II KO K2C8 |
|---|---|
| Target | Cytokeratin 8 |
| Molecular Weight(MW) | 54 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using KRT8 antibody.
Application
Immunofluorescence analysis of A549 cell using KRT8 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of HeLa cell using KRT8 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human esophageal using KRT8 antibody at dilution of 1:100 (x400 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, ICC 1:50 - 1:100, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
