Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8 antibody at 1:500 dilution.Application
Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Cytokeratin 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Cytokeratin 8 (keratin 8)This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Rhesus Monkey, BovineZebrafish (83%), Xenopus laevis (85%), Dog (91%), Pig (91%), Rhesus Monkey (99%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CARD2 antibody CK8 antibody CYK8 antibody K2C8 antibody K8 antibody KO antibody KRT8 antibody cytokeratin-8 antibody type-II keratin Kb8 antibody cytokeratin 8 antibody "keratin type II cytoskeletal 8 antibody" keratin 8 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 8 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Cytokeratin 8 antibody at 1:500 dilution.
Application
Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | H1299 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
