Application
Western blot analysis of D4-DGI with Jurkat cell lysate.| Product Name | D4-GDI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to D4-GDID4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Ly-GDI 602843 P52566 RAP1GN1 GDIA2 D4 LyGDI GDID4 ARHGDIB Ly GDI |
|---|---|
| Target | D4-GDI |
| Tissue Specificity | This antibody is specific for D4-GDI. |
Application
Western blot analysis of D4-DGI with Jurkat cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
Data sheet for OM249349