Application
Immunohistochemical staining of DAPK2 in mouse spleen cells with DAPK2 antibody at 2μg/ml.Application
Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1μg/ml.| Product Name | DAP Kinase 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DAP Kinase 2Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase-containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types of cells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA ofDAPK2 is expressed in multiple human tissues (1). |
| Immunogen | Peptide - amino acids 356-370 of human DAPK2. This sequence is identical to that of mouse. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAPK2 Q9UIK4 DRP-1 23604 DAP Kinase 2 DRP1 DAP Kinase2 DRP 1 |
|---|---|
| Target | DAP Kinase 2 |
| Tissue Specificity | Does not react with DAPK1 |
Application
Immunohistochemical staining of DAPK2 in mouse spleen cells with DAPK2 antibody at 2μg/ml.
Application
Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody at 1μg/ml.| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
