Application
Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A); SHSY5Y (B) whole cell lysates.Application
Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | DARPP-32 (phospho Thr75) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DARPP-32 (phospho Thr75)This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DARPP32 (pT75). The exact sequence is proprietary. |
| Modification | p-Thr75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DARPP-32 PPP1R1B DARPP32 protein phosphatase 1 regulatory (inhibitor) subunit 1B |
|---|---|
| Target | DARPP-32 |
| Tissue Specificity | Recognizes endogenous levels of DARPP32 (pT75) protein. |
Application
Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A); SHSY5Y (B) whole cell lysates.
Application
Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
