Application
WB analysis of rat caudate tissue lysate using DARPP-32 (phospho Thr75) antibody. It shows phosphospecific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr75| Product Name | DARPP-32 (phospho Thr75) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DARPP-32 (phospho Thr75)potent inhibitor of protein phosphatase 1 (PP1); may play a role in maintaining the levels of excitability of NMDA receptors [RGD, Feb 2006] |
| Immunogen | Synthetic phosphopeptide corresponding to an amino acid sequence within DARPP-32 which includes phosphorylated Thr75. |
| Modification | p-Thr75 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blotting: Max Dilution 1/1000 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Rat |
| Predicted Reactivity | Mouse, Monkey, Human, Dog, Chicken, Bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Darpp32 protein phosphatase 1 regulatory (inhibitor) subunit 1B Ppp1r1b Darpp-32 |
|---|---|
| Target | DARPP-32 (phospho Thr75) |
| Tissue Specificity | This antibody recognizes rat DARPP-32, when phosphorylated at threonine 75. |
Application
WB analysis of rat caudate tissue lysate using DARPP-32 (phospho Thr75) antibody. It shows phosphospecific immunolabeling of the ~32 kDa DARPP-32 protein phosphorylated at Thr75| Application Notes | Western Blotting: Max Dilution 1/1000 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | 10mM HEPES pH7.5 containing 0.09% Sodium Azide 0.01% Bovine Serum Albumin 50% Glycerol |
