Application
Immunofluorescence staining of methanol-fixed Hela cells using DARPP-32(Phospho-Thr75) Antibody| Product Name | DARPP-32 (phospho Thr75) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DARPP-32 (Phospho Thr75)Inhibitor of protein-phosphatase 1. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from Human DARPP-32. |
| Modification | p-Thr75 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| Suggested starting dilutions are as follows. ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DARPP-32 DARPP32 FLJ20940 PPP1R1B dopamine and cAMP-regulated neuronal phosphoprotein 32 protein phosphatase 1 regulatory subunit 1B protein phosphatase 1 regulatory (inhibitor) subunit 1B |
|---|---|
| Target | DARPP-32 (Phospho Thr75) |
| Molecular Weight(MW) | 32 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using DARPP-32(Phospho-Thr75) Antibody| Application Notes | Suggested starting dilutions are as follows. ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
