Application
WB analysis of HepG2 cells using DAZ4 antibody at 0.2-1μg/ml.Application
IHC-P analysis of human stomach tissue using DAZ4 antibody at 4.0-8.0μg/ml.Application
IHC-P analysis of human kidney tissue using DAZ4 antibody at 4.0-8.0μg/ml.| Product Name | DAZ4 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DAZ4This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2011] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human DAZ4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Goat: 93%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 92%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PDP1680 DAZ4 PDP1681 |
|---|---|
| Target | DAZ4 |
| Molecular Weight(MW) | 65 KDa (note) |
Application
WB analysis of HepG2 cells using DAZ4 antibody at 0.2-1μg/ml.
Application
IHC-P analysis of human stomach tissue using DAZ4 antibody at 4.0-8.0μg/ml.
Application
IHC-P analysis of human kidney tissue using DAZ4 antibody at 4.0-8.0μg/ml.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
