Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:500| Product Name | DCTN3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DCTN3 (dynactin 3 (p22))This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq] |
| Immunogen | Full length human DCTN3 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (84%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DCTN-22 antibody DCTN22 antibody MGC111190 antibody DCTN3 antibody "dynactin 3 isoform 1 antibody" dynactin complex subunit 22 kDa subunit antibody p22 antibody dynactin light chain antibody dynactin subunit 3 antibody dynactin 3 (p22) antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Centrosome , Kinetochore , Spindle |
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:500| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
