Rabbit Polyclonal antibody to DCUN1D2 (DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae))May contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.
Immunogen
Recombinant protein encompassing a sequence within the center region of human DCUN1D2. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Xenopus laevisMouse (94%), Rat (94%), Xenopus laevis (89%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
C13orf17 antibody FLJ10704 antibody FLJ20092 antibody DCUN1D2 antibody DCUN1 domain-containing protein 2 antibody defective in cullin neddylation protein 1-like protein 2 antibody DCN1-like protein 2 antibody "DCN1 defective in cullin neddylation 1
Molecular Weight(MW)
30 kDa (note) (2)
Application
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using DCUN1D2 antibody at 1:500 dilution.
Positive Control
A549 , HeLa , HepG2 , HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.