Application
DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DDAH2 antibody [N2C3] dilution: 1:500.Application
DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 min. Green: DDAH2 protein stained by DDAH2 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:1000| Product Name | DDAH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DDAH2 (dimethylarginine dimethylaminohydrolase 2)This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDAH2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (95%), Rat (95%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DDAH antibody DDAHII antibody G6a antibody NG30 antibody DDAH2 antibody protein G6a antibody NG-dimethylarginine dimethylamino hydrolase homolog antibody S-phase protein antibody "alternative name: NG30 G6a antibody" dimethylarginine dimethylamin |
|---|---|
| Target | DDAH2 |
| Molecular Weight(MW) | 30 kDa (note) (4) |
Application
DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. DDAH2 antibody [N2C3] dilution: 1:500.
Application
DDAH2 antibody [N2C3] detects DDAH2 protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 min. Green: DDAH2 protein stained by DDAH2 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
