Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using DDEFL1 antibody at 1:500 dilution.| Product Name | DDEFL1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDEFL1 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 3)This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDEFL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (82%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACAP4 antibody, CENTB6 antibody, DDEFL1 antibody, FLJ20199 antibody, UPLC1 antibody, ASAP3 antibody, up-regulated in liver cancer 1 (UPLC1) antibody, ARF6 GTPase-activating protein antibody, protein up-regulated in liver cancer 1 antibody, "arf-GAP with SH |
|---|---|
| Molecular Weight(MW) | 99 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using DDEFL1 antibody at 1:500 dilution.| Positive Control | A549 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
