Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDI1 antibody at 1:100 dilution.Application
Sample(30 ug whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:1000Application
DDI1 antibody [N1C3] detects DDI1 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDI1 protein stained by DDI1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DDI1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DDI1 (DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDI1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus MonkeyMouse (82%), Rat (84%), Rhesus Monkey (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ36017 antibody, DDI1 antibody, "DDI1, DNA-damage inducible 1, homolog 1 antibody", DNA-damage inducible protein 1 antibody, protein DDI1 homolog 1 antibody, DNA-damage inducible 1 homolog 1 (S. cerevisiae) antibody |
|---|---|
| Target | DDI1 |
| Molecular Weight(MW) | 44 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DDI1 antibody at 1:100 dilution.
Application
Sample(30 ug whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:1000
Application
DDI1 antibody [N1C3] detects DDI1 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DDI1 protein stained by DDI1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
