Application
Western blot analysis of anti-DDR1 Antibody (N-term) Pab in placenta lysate. DDR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
DDR1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
DDR1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DDR1 Antibody (N-term) for immunohistochemistry.| Product Name | DDR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DDR1Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. |
| Immunogen | The immunogen corresponding to a region within amino acids 17 and 47 of human DDR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-1:50. For FACS: Use at a dilution of 1:10-1:50. Optimal concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | TRKE CD167 EDDR1 600408 NEP PTK3 PTK3A 780 DDR CAK Q08345 DDR1 NTRK4 RTK6 MCK10 |
|---|---|
| Target | DDR1 |
| Cellular Localization | Plasma membrane |
Application
Western blot analysis of anti-DDR1 Antibody (N-term) Pab in placenta lysate. DDR1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
DDR1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
DDR1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DDR1 Antibody (N-term) for immunohistochemistry.| Positive Control | Placenta lysate. |
|---|---|
| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:1000. For IHC-P: Use at a dilution of 1:10-1:50. For FACS: Use at a dilution of 1:10-1:50. Optimal concentration should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide; Constituents: PBS |
