Application
Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 10% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX19B antibody at 1:200 dilution.| Product Name | DDX19B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX19B (DEAD (Asp-Glu-Ala-As) box polypeptide 19B)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX19B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Chimpanzee, BovineMouse (94%), Rat (94%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DBP5 antibody DDX19 antibody RNAh antibody DDX19B antibody ATP-dependent RNA helicase DDX19 antibody "DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5 yeast homolog) antibody" DEAD-box RNA helicase DEAD5 antibody yeast Dbp5 homolog antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus , nuclear pore complex , Nucleus membrane |
Application
Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 10% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX19B antibody at 1:200 dilution.| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
