Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using DDX3 antibody at 1:250 dilution.Application
U87-MG whole cell and cytoplasm extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal diluted at 1:2000.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal diluted at 1:2000.Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3 antibody at 1:200 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3 antibody at 1:500 dilution.Application
Sample (20 ug of whole cell lysate) A: human ESC 7.5% SDS PAGE diluted at 1:2000| Product Name | DDX3 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX3 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DDX3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Chicken, BovineMouse (98%), Xenopus laevis (97%), Pig (100%), Chicken (98%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DBX antibody, DDX14 antibody, DDX3 antibody, HLP2 antibody, DDX3X antibody, helicase like protein 2 antibody, "DEAD box, X isoform antibody", DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3 antibody, CAP-Rf antibody, helicase-like protein 2 antibody, DEAD/H |
|---|---|
| Molecular Weight(MW) | 73 kDa (note) (3) |
| Cellular Localization | Nucleus speckle , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human normal colon, using DDX3 antibody at 1:250 dilution.
Application
U87-MG whole cell and cytoplasm extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal diluted at 1:2000.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX3 antibody [N3C2], Internal diluted at 1:2000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using DDX3 antibody at 1:200 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DDX3 antibody at 1:500 dilution.
Application
Sample (20 ug of whole cell lysate) A: human ESC 7.5% SDS PAGE diluted at 1:2000| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , U87MG , U87-MG Cytoplasm , Human ESC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
