Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE DDX5 antibody diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DDX5 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using DDX5 antibody at 1:100 dilution.| Product Name | DDX5 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DDX5 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 5)DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDX5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434E109 antibody, DKFZp686J01190 antibody, G17P1 antibody, HLR1 antibody, HUMP68 antibody, p68 antibody, DDX5 antibody, probable ATP-dependent RNA helicase DDX5 antibody, "DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody", |
|---|---|
| Molecular Weight(MW) | 69 kDa (note) (4) |
| Cellular Localization | Nucleus , nucleolus |
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE DDX5 antibody diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DDX5 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using DDX5 antibody at 1:100 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
