Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1 antibody at 1:500 dilution.Application
Sample (20 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
DECR1 antibody [N1C3] detects DECR1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DECR1 protein stained by DECR1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DECR1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | DECR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DECR1 (2,4-dienoyl CoA reductase 1, mitochondrial)This gene encodes an accessory enzyme which participates in the beta-oxidation and metabolism of unsaturated fatty enoyl-CoA esters. [provided by RefSeq] |
| Immunogen | Full length human DECR1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (85%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DECR antibody, NADPH antibody, SDR18C1 antibody, DECR1 antibody, "short chain dehydrogenase/reductase family 18C, member 1 antibody", "2,4-dienoyl-CoA reductase, mitochondrial antibody", 4-enoyl-CoA reductase antibody, "2,4-dienoyl CoA reductase 1, mitocho |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1 antibody at 1:500 dilution.
Application
Sample (20 µg of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
DECR1 antibody [N1C3] detects DECR1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DECR1 protein stained by DECR1 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DECR1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver , Rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
