Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DECR2 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000Application
DECR2 antibody detects DECR2 protein at peroxisome by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DECR2 protein stained by DECR2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DECR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DECR2 (2,4-dienoyl CoA reductase 2, peroxisomal)Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Has activity towards short and medium chain 2,4-dienoyl-CoAs, but also towards 2,4,7,10,13,16,19-docosaheptaenoyl-CoA, suggesting that it does not constitute a rate limiting step in the peroxisomal degradation of docosahexaenoic acid. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DECR2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Rat, Rhesus MonkeyRat (80%), Rhesus Monkey (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PDCR antibody, SDR17C1 antibody, DECR2 antibody, "2,4-dienoyl-CoA reductase 2 antibody", "short chain dehydrogenase/reductase family 17C, member 1 antibody", "peroxisomal 2,4-dienoyl-CoA reductase antibody", "2,4-dienoyl CoA reductase 2, peroxisomal antibo |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) (2) |
| Cellular Localization | Peroxisome |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using DECR2 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:1000
Application
DECR2 antibody detects DECR2 protein at peroxisome by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DECR2 protein stained by DECR2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
