Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAG kinase gamma antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DAG kinase gamma antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | DGKG antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DGKG (diacylglycerol kinase, gamma 90kDa)This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DGKG. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (95%), Rat (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DAGK3 antibody, DGK-GAMMA antibody, MGC104993 antibody, MGC133330 antibody, DGKG antibody, diacylglycerol kinase gamma antibody, DAG kinase gamma antibody, diacylglyerol kinase gamma antibody, diglyceride kinase gamma antibody, "diacylglycerol kinase, gamm |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using DAG kinase gamma antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DAG kinase gamma antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , HeLa , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
