Application
DHX9 antibody detects DHX9 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DHX9 antibody diluted at 1:1000.Application
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. DHX9 antibody diluted at 1:500.Application
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. DHX9 antibody diluted at 1:500.Application
DHX9 antibody detects DHX9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DHX9 protein stained by DHX9 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | DHX9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DHX9DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DHX9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | LKP NDHII DDX9 DEAH (Asp-Glu-Ala-His) box helicase 9 NDH2 RHA |
|---|---|
| Target | DHX9 |
| Molecular Weight(MW) | 141 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , nucleolus |
Application
DHX9 antibody detects DHX9 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DHX9 antibody diluted at 1:1000.
Application
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft. DHX9 antibody diluted at 1:500.
Application
DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in CAL 27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded CAL 27 xenograft. DHX9 antibody diluted at 1:500.
Application
DHX9 antibody detects DHX9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DHX9 protein stained by DHX9 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa , HeLa nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
