Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody diluted at 1:500.Application
DIAPH3 antibody detects DIAPH3 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. DIAPH3 antibody diluted at 1:400.| Product Name | DIAPH3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DIAPH3 (diaphanous-related formin 3)Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | BovineBovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | diaphanous homolog 3 (Drosophila) DIA2 diap3 AUNA1 NSDAN mDia2 DRF3 AN |
|---|---|
| Target | DIAPH3 |
| Molecular Weight(MW) | 137 kDa (note) (7) |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody diluted at 1:500.
Application
DIAPH3 antibody detects DIAPH3 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. DIAPH3 antibody diluted at 1:400.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
