Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody [C1C3] diluted at 1:500.Application
DIAPH3 antibody [C1C3] detects DIAPH3 protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. DIAPH3 antibody [C1C3] dilution: 1:100.| Product Name | DIAPH3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DIAPH3 (diaphanous homolog 3 (Drosophila))Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (84%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp434C0931 antibody DKFZp686A13178 antibody DRF3 antibody FLJ34705 antibody diap3 antibody mDia2 antibody DIAPH3 antibody diaphanous-related formin-3 antibody diaphanous-related formin 3 antibody "auditory neuropathy autosomal dominant 1 antib |
|---|---|
| Target | DIAPH3 |
| Molecular Weight(MW) | 137 kDa (note) (6) |
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody [C1C3] diluted at 1:500.
Application
DIAPH3 antibody [C1C3] detects DIAPH3 protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. DIAPH3 antibody [C1C3] dilution: 1:100.| Positive Control | mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
