Application
Western Blot Analysis of Dio-1 expression with Jurkat Cell Lysate.| Product Name | Dio-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dio-1Apoptosis plays an important role in many cellular processes. Dio-1 (Death Inducer-O bliterator-1), a putative transcription factor, has been indicated to function in the onset of apoptosis. Dio-1 expression is upregulated when an apoptosis is detected. It translocates to the nucleus, and activates apoptosis during limb development. Dio-1 expression can be suppressed by caspase inhibitors and Bcl-2 expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes the 62 kDa human and mouse Dio-1 in Western blot analysis. Jurkat cell lysate and mouse small intestine tissue lysate can be used as positive controls.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | dJ885L7.8 DIDO3 DATF1 MGC16140 DIDO1 FLJ11265 DIO1 DKFZp434P1115 C20orf158 KIAA0333 DIO-1 BYE1 DIDO2 DIO 1 |
|---|---|
| Target | Dio-1 |
| Tissue Specificity | This antibody is specific for Dio-1. |
| Cellular Localization | Translocates to the nucleus after pro-apoptotic stimuli. |
Application
Western Blot Analysis of Dio-1 expression with Jurkat Cell Lysate.| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes the 62 kDa human and mouse Dio-1 in Western blot analysis. Jurkat cell lysate and mouse small intestine tissue lysate can be used as positive controls.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
