Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DIP13B antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DIP13B antibody at 1:100 dilution.| Product Name | DIP13B antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DIP13B (adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2)Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DIP13B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (92%), Rat (91%), Pig (91%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DIP13B antibody FLJ10659 antibody APPL2 antibody "adapter protein containing PH domain PTB domain and leucine zipper motif 2 antibody" DIP13 beta antibody DCC-interacting protein 13-beta antibody "adaptor protein phosphotyrosine interaction PH dom |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (4) |
| Cellular Localization | Early endosome membrane , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using DIP13B antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DIP13B antibody at 1:100 dilution.| Positive Control | NT2D1 , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
