Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] diluted at 1:5000 and competitor's antibody (SC-13974) diluted by 1:200.Application
Dvl-2 antibody [N1N3] detects Dvl-2 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Dishevelled 2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Dishevelled 2 (dishevelled, dsh homolog 2 (Drosophila))This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dvl-2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (94%), Rat (92%), Rhesus Monkey (96%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DVL2 antibody segment polarity protein dishevelled homolog DVL-2 antibody dishevelled-2 antibody DSH homolog 2 antibody dishevelled 2 (homologous to Drosophila dsh) antibody "dishevelled dsh homolog 2 (Drosophila) antibody" |
|---|---|
| Target | Dvl-2 |
| Molecular Weight(MW) | 79 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3] diluted at 1:5000 and competitor's antibody (SC-13974) diluted by 1:200.
Application
Dvl-2 antibody [N1N3] detects Dvl-2 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
