Application
Sample(30 µg whole cell lysate) A: MOLT4 B: Raji 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2 antibody at 1:100 dilution.| Product Name | Dishevelled 2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Dishevelled 2 (dishevelled, dsh homolog 2 (Drosophila))This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Dishevelled 2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rhesus Monkey, BovineRat (99%), Zebrafish (80%), Xenopus laevis (85%), Rhesus Monkey (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DVL2 antibody segment polarity protein dishevelled homolog DVL-2 antibody dishevelled-2 antibody DSH homolog 2 antibody dishevelled 2 (homologous to Drosophila dsh) antibody "dishevelled dsh homolog 2 (Drosophila) antibody" |
|---|---|
| Molecular Weight(MW) | 79 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample(30 µg whole cell lysate) A: MOLT4 B: Raji 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using Dishevelled 2 antibody at 1:100 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
