Application
Sample(30 μg of whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed Hep3B, using DLD antibody at 1:100 dilution.| Product Name | DLD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DLD (dihydrolipoamide dehydrogenase)This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Chicken, BovineXenopus laevis (94%), Dog (98%), Pig (99%), Chicken (93%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DLDH antibody E3 antibody GCSL antibody LAD antibody PHE3 antibody DLD antibody "E3 component of pyruvate dehydrogenase complex 2-oxo-glutarate complex branched chain keto acid dehydrogenase complex antibody" lipoamide reductase antibody lipoamid |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (4) |
| Cellular Localization | Mitochondrion matrix |
Application
Sample(30 μg of whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using DLD antibody at 1:100 dilution.| Positive Control | 293T , mouse brain , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
