Application
Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 ug/ml.Application
Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 ug/mL.Application
Western blot analysis of SAPAP1 in human brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 ug/ml.| Product Name | DLGAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DLGAP1SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Disks largeassociated protein1 DAP1 DAP-1 Disks large-associated protein 1 SAP90/PSD-95-associated protein 1 Disks largeassociated protein 1 SAPAP1 SAP90/PSD95associated protein 1 GKAP SAP90/PSD95associated protein1 DLGAP1 DAP 1 |
|---|---|
| Target | DLGAP1 |
| Tissue Specificity | This antibody is specific for DLGAP1 |
Application
Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 ug/ml.
Application
Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 ug/mL.
Application
Western blot analysis of SAPAP1 in human brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
