Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed A431, using DLST antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST antibody at 1:500 dilution.| Product Name | DLST antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DLST (dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex))The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human DLST. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (95%), Zebrafish (87%), Xenopus laevis (90%), Pig (95%), Chicken (81%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DLTS antibody DLST antibody E2K antibody "dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex mitochondrial antibody" OGDC-E2 antibody dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutara |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed A431, using DLST antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST antibody at 1:500 dilution.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
