Application
Western blot analysis of LIG1 in cell lysate from HeLa cells using at 1:1000 dilution.| Product Name | DNA ligase I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DNA ligase ILIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. |
| Immunogen | Synthetic peptide, corresponding to amino acids 51-68 (SPVKRPGRKAARVLGSEG) of Human Lig1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 126391 MGC130025 P18858 3978 Ligase 1 MGC117397 LIG1 Ligase1 |
|---|---|
| Target | DNA ligase I |
| Cellular Localization | Nuclear |
Application
Western blot analysis of LIG1 in cell lysate from HeLa cells using at 1:1000 dilution.| Positive Control | HeLa cells |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. Predicted molecular weight: 100 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS, 0.1mg/ml BSA |
