Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA ligase IV antibody at 1:500 dilution.Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.| Product Name | DNA ligase IV antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to DNA ligase IV (ligase IV, DNA, ATP-dependent)The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase IV. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Sheep, BovineSheep (83%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LIG4 antibody DNA ligase 4 antibody polynucleotide ligase antibody sealase antibody DNA repair enzyme antibody DNA ligase IV antibody polydeoxyribonucleotide synthase [ATP] 4 antibody DNA joinase antibody "ligase IV DNA ATP-dependent antibody" |
|---|---|
| Molecular Weight(MW) | 104 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using DNA ligase IV antibody at 1:250 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DNA ligase IV antibody at 1:500 dilution.
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase IV antibody diluted at 1:500.| Positive Control | U87-MG , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
